Menthol and Fatty Acid-Based Hydrophobic Deep Eutectic Solvents as Media for Enzyme Activation

This research aims to provide insights into the biological efficacy of a newly formed hydrophobic deep eutectic solvent (DES). A DES based on menthol was successfully synthesized with fatty acids. The DESs’ properties as enzyme activators were examined against neat counterpart. menthol:decanoic acid (1:1) combination showed improved thermal stability, strong catalytic activity, and reusability for up four subsequent cycles under ideal conditions (pH 7.0, 40 °C 2 h). replaced hexane in ester synthesis, where RNL@DES5 better conversion compared RNL. study demonstrated promising applications DESs non-aqueous organic reactions.